Mass distribution, polydispersity and focusing properties of carrier ampholytes for IEF. IV: pH 6-8 intervals

Four commercial brands of carrier ampholytes (Ampholine, Pharmalyte, Servalyt, Bio-Lyte), in the pH 6–8 range, have been analyzed by a 2-D technique based on preparative Rotofor fractionation followed by CE-MS of 10 out of 20 fractions harvested, in the second dimension. The findings: Ampholine (pH 6–8) contains 80 different Mr compounds, in the Mr interval 216–979 Da, for a total of 326 isoforms. Bio-Lyte (pH 6–8) consists of 62 different Mr species, in the Mr range 341–1048 Da, for a total of 237 isoforms. Servalyt (pH 6–8) is made of 126 different Mr compounds, in the Mr interval 240–785 Da, for a total of 703 isoforms. Pharmalyte (pH 5–8) comprises 123 amphoteres, in the Mr range of 221–992 Da, for a total of 476 isoforms. Pharmalyte appears to be the best brand, with a good proportion of species focusing sharply at their pI position and relatively few ’poor' species, distributed along the entire pH gradient. General conclusions are drawn on the properties of all the pH intervals explored in this series of investigations and some guidelines for possible synthetic routes ameliorating the neutral and alkaline pH intervals are discussed