In the present paper we describe a novel approach for DNA microarray reading. We used an intensified CCD camera with picosecond time resolution either for single shot image acquisition or for time resolved measurements. We characterized the time resolution of the system. Then, we demonstrated that two fluorescent markers used for oligonucleotide labeling can be discriminated in the time domain instead of the spectral one. Finally, we applied our system to read a PNA microarray.

DNA microarray reading by an intensified CCD camera with picosecond time resolution

VALENTINI, GIANLUCA;CUBEDDU, RINALDO;D'ANDREA, COSIMO;PIFFERI, ANTONIO GIOVANNI;TARONI, PAOLA;TORRICELLI, ALESSANDRO
2000

Abstract

In the present paper we describe a novel approach for DNA microarray reading. We used an intensified CCD camera with picosecond time resolution either for single shot image acquisition or for time resolved measurements. We characterized the time resolution of the system. Then, we demonstrated that two fluorescent markers used for oligonucleotide labeling can be discriminated in the time domain instead of the spectral one. Finally, we applied our system to read a PNA microarray.
Proceedings Biomedical Optical Spectroscopy and Diagnostics
155752632X
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11311/259518
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