Microfluidic SERS platform integrating in situ gold nanoparticles for bio film detection

Komagataeibacter xylinus is a model organism for bacterial cellulose (BC) production, relevant to biomaterials and biofilm research. Rapid, non-destructive monitoring of biochemical variations during biofilm formation is essential for optimizing BC synthesis. This study presents a proof-of-concept microfluidic SERS platform for real-time detection of metabolites released during K. xylinus biofilm growth. Gold nanoparticles (AuNPs) were synthesized via the Turkevich method and an in-situ biological route, then integrated into a laser-etched PDMS microreactor. Multivariate analysis (PCA) of SERS spectra revealed temporal biochemical shifts reflecting biofilm dynamics. The system demonstrates the feasibility of combining SERS, microfluidics, and PCA for dynamic monitoring of BC production and offers potential for broader bioprocess applications.