One of the major drawbacks of Time-Correlated Single Photon Counting (TCSPC) is generally represented by pile-up distortion, that strongly bounds the maximum acquisition speed to few percents of the laser excitation rate. Based on a previous theoretical analysis, recently we presented the first low-distortion and high-speed TCSPC system capable of overcoming the pile-up limitation by perfectly matching the Single-Photon Avalanche Diode (SPAD) dead time to the laser period. In this work, we validate the proposed system in a standard fluorescence measurement by comparing experimental data with the reference theoretical framework. As a result, a count rate of 32 Mcps was achieved with a single-channel system still observing a negligible lifetime distortion.

Above pile-up fluorescence microscopy with a 32 Mc/s single-channel time-resolved SPAD system

Serena Farina;Ivan Labanca;Giulia Acconcia;Alberto Ghezzi;Andrea Farina;Cosimo D'Andrea;Ivan Rech
2022

Abstract

One of the major drawbacks of Time-Correlated Single Photon Counting (TCSPC) is generally represented by pile-up distortion, that strongly bounds the maximum acquisition speed to few percents of the laser excitation rate. Based on a previous theoretical analysis, recently we presented the first low-distortion and high-speed TCSPC system capable of overcoming the pile-up limitation by perfectly matching the Single-Photon Avalanche Diode (SPAD) dead time to the laser period. In this work, we validate the proposed system in a standard fluorescence measurement by comparing experimental data with the reference theoretical framework. As a result, a count rate of 32 Mcps was achieved with a single-channel system still observing a negligible lifetime distortion.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11311/1189958
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