In vitro cell/tissue culture represents a widespread methodology to investigate pathophysiology with good cost/benefit ratio, in compliance with the 3R principles. Cell differentiation, co-culture, conditioning under perfusion and recover/reuse are among the key limitations of cell culture, and currently available systems are unsuitable to perform all of them. We developed and are now validating a novel system, allowing compartmentalized cultures, visualization in real time of the culture growth, and recover and reuse of the biological material for subsequent analysis.

IN VITRO VALIDATION OF A NOVEL BICOMPARTMENTAL CULTURE SYSTEM.

L. P. Coppadoro;G. B. Fiore;M. Soncini;
2019-01-01

Abstract

In vitro cell/tissue culture represents a widespread methodology to investigate pathophysiology with good cost/benefit ratio, in compliance with the 3R principles. Cell differentiation, co-culture, conditioning under perfusion and recover/reuse are among the key limitations of cell culture, and currently available systems are unsuitable to perform all of them. We developed and are now validating a novel system, allowing compartmentalized cultures, visualization in real time of the culture growth, and recover and reuse of the biological material for subsequent analysis.
2019
cell culture, differentiation, co-culture, bicompartmental, recover, reuse, real time imaging
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1121465
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