Single-molecule techniques probe the spectroscopic properties of biomolecules one molecule a time. These approaches allow detecting and quantifying subpopulations which would be masked in ensemble measurements. Single-molecule FRET (smFRET), which indirectly measures the distance between fluorophores, has been extensively used to characterize the conformation of biomolecules or molecular interactions in complex biomolecular assemblies among many applications. FRET efficiency is typically computed ratiometrically from photon counts detected in two spectral channels.
A 16 Channel Spad Array for High-Throughput Tcspc Measurements of Single-Molecule FRET of Freely Diffusing Molecules
PERONIO, PIETRO;RECH, IVAN;GULINATTI, ANGELO;GHIONI, MASSIMO ANTONIO;
2016-01-01
Abstract
Single-molecule techniques probe the spectroscopic properties of biomolecules one molecule a time. These approaches allow detecting and quantifying subpopulations which would be masked in ensemble measurements. Single-molecule FRET (smFRET), which indirectly measures the distance between fluorophores, has been extensively used to characterize the conformation of biomolecules or molecular interactions in complex biomolecular assemblies among many applications. FRET efficiency is typically computed ratiometrically from photon counts detected in two spectral channels.File in questo prodotto:
| File | Dimensione | Formato | |
|---|---|---|---|
|
Biophysj 2016 Ingargiola-1.pdf
Accesso riservato
Descrizione: Abstract
:
Publisher’s version
Dimensione
41.89 kB
Formato
Adobe PDF
|
41.89 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
