Laser speckle contrast imaging (LSCI) has emerged as a valuable tool for cerebral blood flow (CBF) imaging. We present a multi-exposure laser speckle imaging (MESI) method which uses a high-frame rate acquisition with a negligible inter-frame dead time to mimic multiple exposures in a single-shot acquisition series. Our approach takes advantage of the noise-free readout and high-sensitivity of a complementary metal-oxide-semiconductor (CMOS) single-photon avalanche diode (SPAD) array to provide real-time speckle contrast measurement with high temporal resolution and accuracy. To demonstrate its feasibility, we provide comparisons between in vivo measurements with both the standard and the new approach performed on a mouse brain, in identical conditions.

High-speed multi-exposure laser speckle contrast imaging with a single-photon counting camera

BRONZI, DANILO;VILLA, FEDERICA ALBERTA;TOSI, ALBERTO;ZAPPA, FRANCO;
2015

Abstract

Laser speckle contrast imaging (LSCI) has emerged as a valuable tool for cerebral blood flow (CBF) imaging. We present a multi-exposure laser speckle imaging (MESI) method which uses a high-frame rate acquisition with a negligible inter-frame dead time to mimic multiple exposures in a single-shot acquisition series. Our approach takes advantage of the noise-free readout and high-sensitivity of a complementary metal-oxide-semiconductor (CMOS) single-photon avalanche diode (SPAD) array to provide real-time speckle contrast measurement with high temporal resolution and accuracy. To demonstrate its feasibility, we provide comparisons between in vivo measurements with both the standard and the new approach performed on a mouse brain, in identical conditions.
(030.5260) Photon counting; (040.1345) Avalanche photodiodes (APDs); (110.6150) Speckle imaging; (300.6480) Spectroscopy, speckle; sezele
File in questo prodotto:
File Dimensione Formato  
2015 - Dragojevic - High-speed multi-exposure laser speckle contrast imaging with a single-photon counting camera.pdf

accesso aperto

Descrizione: Articolo principale
: Publisher’s version
Dimensione 1.42 MB
Formato Adobe PDF
1.42 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11311/971974
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 36
  • ???jsp.display-item.citation.isi??? 31
social impact