In this work, a new method to functionalize a gold surface by dip coating with a functional copolymer is presented. The coating procedure is simple, robust and can be accomplished in less than one hour. Atomic force microscopy (AFM) scratch tests reveal the presence of a homogeneous polymer coating with a thickness of 2.5nm. X-ray photoemission spectroscopy spectra from C1s, N1s and O1s levels present the typical fingerprints of the polymeric overlayer, i.e. the characteristic peaks from CNC   O and NC   O groups. Surface plasmon resonance (SPR) binding assays were used to check the coating functional properties. Immobilization of heparin to SPR gold surfaces functionalized with copoly(DMA-NAS-MAPS)- followed by binding analysis with the well known heparin binding protein fibroblast growth factor 2 yield binding kinetic parameters comparable to those obtained with commercially available carboxymethyl dextran- functionalized sensorchips, thus confirming the great potential of the proposed technique.

Functionalization of gold surfaces with copoly(DMA-NAS-MAPS) by dip coating: Surface characterization and hybridization tests

PETTI, DANIELA;TORTI, ANDREA MARIO;ALBISETTI, EDOARDO;BERTACCO, RICCARDO;
2014

Abstract

In this work, a new method to functionalize a gold surface by dip coating with a functional copolymer is presented. The coating procedure is simple, robust and can be accomplished in less than one hour. Atomic force microscopy (AFM) scratch tests reveal the presence of a homogeneous polymer coating with a thickness of 2.5nm. X-ray photoemission spectroscopy spectra from C1s, N1s and O1s levels present the typical fingerprints of the polymeric overlayer, i.e. the characteristic peaks from CNC   O and NC   O groups. Surface plasmon resonance (SPR) binding assays were used to check the coating functional properties. Immobilization of heparin to SPR gold surfaces functionalized with copoly(DMA-NAS-MAPS)- followed by binding analysis with the well known heparin binding protein fibroblast growth factor 2 yield binding kinetic parameters comparable to those obtained with commercially available carboxymethyl dextran- functionalized sensorchips, thus confirming the great potential of the proposed technique.
Surface functionalization
File in questo prodotto:
File Dimensione Formato  
Sensors ad Actuators 13.pdf

Accesso riservato

: Post-Print (DRAFT o Author’s Accepted Manuscript-AAM)
Dimensione 2.25 MB
Formato Adobe PDF
2.25 MB Adobe PDF   Visualizza/Apri
Functionalization of gold surfaces with copoly DMA-NAS-MAPS_11311-759070_Bertacco.pdf

accesso aperto

: Post-Print (DRAFT o Author’s Accepted Manuscript-AAM)
Dimensione 1.82 MB
Formato Adobe PDF
1.82 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11311/759070
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
social impact