Three recipes are presented to make tissue constituent-equivalent phantoms of water and lipids. Different approaches to prepare the emulsion are proposed. Nature phantoms are made using no emulsifying agent, but just a professional disperser; instead Agar and Triton phantoms are made using agar or Triton X-100, respectively, as agents to emulsify water and lipids. Different water-to-lipid ratios ranging from 30\% to 70\% by mass were tested. A broadband time-resolved diffuse optical spectroscopy system was used to characterize the phantoms in terms of optical properties and composition. For some water/lipid ratios the emulsion fails or the phantom has limited lifetime, but in most cases the recipes provide phantoms with a high degree of homogeneity [coefficient of variation (CV) of 4.6\% and 1.5\% for the absorption and reduced scattering coefficient, respectively] and good reproducibility (CV of 8.3\% and 12.4\% for absorption and reduced scattering coefficient, respectively).

Recipes to make organic phantoms for diffusive optical spectroscopy.

QUARTO, GIOVANNA;PIFFERI, ANTONIO GIOVANNI;BARGIGIA, ILARIA;FARINA, ANDREA;CUBEDDU, RINALDO;TARONI, PAOLA
2013-01-01

Abstract

Three recipes are presented to make tissue constituent-equivalent phantoms of water and lipids. Different approaches to prepare the emulsion are proposed. Nature phantoms are made using no emulsifying agent, but just a professional disperser; instead Agar and Triton phantoms are made using agar or Triton X-100, respectively, as agents to emulsify water and lipids. Different water-to-lipid ratios ranging from 30\% to 70\% by mass were tested. A broadband time-resolved diffuse optical spectroscopy system was used to characterize the phantoms in terms of optical properties and composition. For some water/lipid ratios the emulsion fails or the phantom has limited lifetime, but in most cases the recipes provide phantoms with a high degree of homogeneity [coefficient of variation (CV) of 4.6\% and 1.5\% for the absorption and reduced scattering coefficient, respectively] and good reproducibility (CV of 8.3\% and 12.4\% for absorption and reduced scattering coefficient, respectively).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/748197
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