Affinity chromatography is among the most widely used methodologies for the purification of proteins. Despite of this, a molecular understanding of its working principles is essentially still missing, mostly because of the difficulty of disentangling the contribution of the different components responsible for the affinity interaction. Molecular modelling, which has greatly progressed in the last years, offers a chance to improve greatly our understanding of this complex process. In this perspective it will be discussed how this is possible and what are the most likely rewards from this effort. Several examples of recent progress in the field will be given as well as a list of open problems and challenges.
Molecular modelling of the affinity chromatography of proteins: Status and perspectives
CAVALLOTTI, CARLO ALESSANDRO;SALVALAGLIO, MATTEO
2012-01-01
Abstract
Affinity chromatography is among the most widely used methodologies for the purification of proteins. Despite of this, a molecular understanding of its working principles is essentially still missing, mostly because of the difficulty of disentangling the contribution of the different components responsible for the affinity interaction. Molecular modelling, which has greatly progressed in the last years, offers a chance to improve greatly our understanding of this complex process. In this perspective it will be discussed how this is possible and what are the most likely rewards from this effort. Several examples of recent progress in the field will be given as well as a list of open problems and challenges.| File | Dimensione | Formato | |
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Cavallotti_analytical chemistry_co6_2012.pdf
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