The aim of this work was to investigate innovative reaction media such as ionic liquids for enzymatic phospholipids transformations in order to get a higher selectivity in the enzymatic transphosphatidylation reactions of natural phosphatidylcholine catalyzed by phospholipase D (PLD) from Streptomyces PMF. The stability and the activity of the PLD in the ionic liquid [BMIM][PF6] was tested. This ionic liquid used as a cosolvent in the synthesis of phosphatidylserine lead surprisingly to the almost quantitative suppression of unwanted hydrolytic side reaction, thus providing a great improvement in the final product purification.

Improvements in the enzymatic synthesis of phosphatidylserine employing ionic liquids

D'ARRIGO, PAOLA;CERIOLI, LORENZO;TESSARO, DAVIDE;MELE, ANDREA
2012-01-01

Abstract

The aim of this work was to investigate innovative reaction media such as ionic liquids for enzymatic phospholipids transformations in order to get a higher selectivity in the enzymatic transphosphatidylation reactions of natural phosphatidylcholine catalyzed by phospholipase D (PLD) from Streptomyces PMF. The stability and the activity of the PLD in the ionic liquid [BMIM][PF6] was tested. This ionic liquid used as a cosolvent in the synthesis of phosphatidylserine lead surprisingly to the almost quantitative suppression of unwanted hydrolytic side reaction, thus providing a great improvement in the final product purification.
Phospholipase D; Transphosphatidylation; Ionic liquid; Phosphatidylserine
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/677944
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