A combined chemical-physical decellularization protocol for whole full-thickness bladder decellularization was developed, representing the first step to the attainment of a biological-derived scaffold for bladder regeneration. Decellularization was obtained through a dynamic perfusion system, allowing cyclic organ mechanical distention aimed at reducing the tissue exposure time to the decellularising agents. Histological analysis was combined with biomechanical characterization, to evaluate the effectiveness of the protocol in preserving in vivo-like tissue composition, 3D architecture and biomechanical behaviour of the acellular organ. The analysis was focused on the retaining of desirable structural and functional components in the acellular matrix. Biomechanical properties were first evaluated at the mesoscale level, then, the whole organ specific distention properties were investigated through ex vivo filling cystometry curve analysis.

A COMPREHENSIVE BIOMECHANICAL ANALYSIS OF A BLADDER ACELLULAR MATRIX DERIVED FROM WHOLE FULL-THICKNESS RABBIT BLADDER

CONSOLO, FILIPPO;FIORE, GIANFRANCO BENIAMINO;SONCINI, MONICA
2012-01-01

Abstract

A combined chemical-physical decellularization protocol for whole full-thickness bladder decellularization was developed, representing the first step to the attainment of a biological-derived scaffold for bladder regeneration. Decellularization was obtained through a dynamic perfusion system, allowing cyclic organ mechanical distention aimed at reducing the tissue exposure time to the decellularising agents. Histological analysis was combined with biomechanical characterization, to evaluate the effectiveness of the protocol in preserving in vivo-like tissue composition, 3D architecture and biomechanical behaviour of the acellular organ. The analysis was focused on the retaining of desirable structural and functional components in the acellular matrix. Biomechanical properties were first evaluated at the mesoscale level, then, the whole organ specific distention properties were investigated through ex vivo filling cystometry curve analysis.
File in questo prodotto:
File Dimensione Formato  
Abstract_Consolo_ESB_2012.pdf

Accesso riservato

: Altro materiale allegato
Dimensione 2.45 MB
Formato Adobe PDF
2.45 MB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/665136
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact