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|Titolo:||Fractal behavior of spontaneous neurotransmitter release: fromsingle-synapse to whole-cell recordings|
|Autori interni:||LAMANNA, JACOPO|
SIGNORINI, MARIA GABRIELLA
|Data di pubblicazione:||2011|
|Abstract:||Spontaneous release of neurotransmitter vesicles at brain chemical synapses has been deeply investigated in the last decades at several levels. First and second order statistics have been widely adopted as a tool for assessing, inter-alia, dependence of spontaneous release on the concentration of ionic species in the intra- or extra-cellular environment. Furthermore, several studies demonstrated that spontaneous release exhibits fractal, and generally non purely random, behavior. Most experimental work on this topic exploits population whole-cell patch-clamp recordings in order to acquire post-synaptic currents elicited by neurotransmitter release into the synaptic cleft. Since several synapses merge on the dendritic arbor of a single neuronal cell, whole-cell recordings of miniature excitatory post-synaptic currents (mEPSCs) implies the temporal superimposition of releasing events from all active synapses on the arbor. This limitation can be overcome by exploiting the loose-patch clamp technique on single synapses, thus acquiring spontaneous release events from individual synapses. Here, we present results obtained by applying well-established methods for the quantification of fractal behavior in the series of mEPSCs acquired through the use of both whole-cell and single-synapse loose-patch recording techniques on hippocampal neurons and synapses. Our long- term aim is to get a better understanding of the release process and of the mechanisms of neuronal integration when the information is coming from several simultaneously active synaptic sites.|
|Appare nelle tipologie:||04.1 Contributo in Atti di convegno|
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