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|Titolo:||Computational modeling for the optimization of a cardiogenic 3D bioprocess of encapsulated embryonic stem cells.|
|Autori interni:||CONSOLO, FILIPPO|
REDAELLI, ALBERTO CESARE LUIGI
|Data di pubblicazione:||2012|
|Rivista:||BIOMECHANICS AND MODELING IN MECHANOBIOLOGY|
|Abstract:||We present a computational ﬂuid dynamics (CFD)-based model aimed at the identiﬁcation of optimized culture conditions promoting efﬁcient cardiogenesis of hydrogel-bead-encapsulated embryonic stem cells (ESCs) within a rotating bioreactor. The numerical approach, integrating diffusion, convection, and multiphase ﬂuid dynamics calculations, allowed to evaluate (i) the microgravity motion of the ﬂoating beads, (ii) the O2 delivery to the cells, also (iii) taking into account the cellular O2 consumption, as a function of different rotation speeds of the breeding chamber. According to our results, a 25 rpm rotation (i) enhances an adequate mixing of the cell carriers, avoiding sedimentation and excessive packing, also maintaining a quite homogeneous distribution of the suspended beads and (ii) imparts a proper cellular O2 supply, providing cells close to a normoxia condition. The bioreactor working conditions derived from the numerical analysis allowed the attainment of in vitro long- term cell viability maintenance, supporting efﬁcient large-scale generation of ESC-derived cardiomyocytes (ESC-DCs) through a chemical-based conditioning bioprocess. In conclusion, we demonstrated the feasibility of using CFD-based tools, as a reliable and cost-effective strategy to assist the design of a 3D cardiogenic bioprocess.|
|Appare nelle tipologie:||01.1 Articolo in Rivista|
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