Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1 – 9X10-3 glucose (R-2=0.9997) with a sensitivity of 1.11 muA/mM and a limit of detection of 2.5X10-6 M (S/N=3). The uncertainty of repeatability was 2% (RSD%, n=30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD%=1%, n=3).

Nylon Nanofibrous Biosensors for Glucose Determination

BIANCO, ANDREA;BERTARELLI, CHIARA;
2010-01-01

Abstract

Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1 – 9X10-3 glucose (R-2=0.9997) with a sensitivity of 1.11 muA/mM and a limit of detection of 2.5X10-6 M (S/N=3). The uncertainty of repeatability was 2% (RSD%, n=30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD%=1%, n=3).
2010
Electrospinning; Nylon-6; glucose oxidase; nanofibrous membrane; amperometric detection; membranes; biosensors
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/574140
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