Methods and system for detecting peptide peaks in HPLC-MS signals

The present invention relates to a method, a computer tool and a system for detecting peptide peaks in measurement signals generated by HPLC-MS instruments. The method comprises the steps of: providing an intensity column vector (SIC) representative of the elution values at a specific mass value (m/z) of the measurement signal; performing a wavelet decomposition of such values of said intensity column vector (SIC) to generate a first processed vector representative of the smoothed intensity values of the intensity column vector (SIC); providing a threshold value (S) and performing a wavelet decomposition as a function of the value of said threshold (S) to generate the second processed vector representative of said intensity column vector (SIC) cleaned of any oscillations generated by stochastic noise (e(t)); processing said first processed vector and said second processed vector to generate a third processed vector identifying the baseline value of said intensity column vector (SIC); processing said intensity column vector (SIC) and said second and third vectors to generate e filtered vector identifying any peak value (zi) below the background noise. Method and system for detecting peptide peaks in HPLC-MS signals