An Advanced Culture System for Coronary Bloodstream-Induced Stimuli Replication in CABG Applications

Coronary Artery Disease (CAD) remains a leading cause of mortality, highlighting the need for improved Coronary Artery Bypass Grafting (CABG) solutions. While autologous grafts are widely used, their limited availability and high failure rates drive the search for alternatives. Traditional preclinical models often fail to accurately replicate human hemodynamics. This study presents the development of an advanced in vitro culture system designed to mimic coronary flow dynamics and support vascular graft maturation. The system integrates automated rotational seeding and multi-sample testing, ensuring standardized conditions. A control unit regulates fluid flow and biological stimuli, while a hydraulic circuit reproduces pulsatile coronary flow. This platform offers a controlled alternative to animal models, advancing vascular tissue engineering and CABG research.