Scattering correction for nonlinear excitation imaging through implanted micro-optics

For the direct application of non-linear excitation in the clinical field one needs to tackle with optical aberrations and the scattering arising from the tissue. We implemented and tested a methodology for reducing the first ones, mainly spherical aberrations, by means of implantable high NA lenses microlenses, which allow to achieve an in-situ focalization of the laser beam. However, tissue scattering must be corrected by directly measuring the scattered field point spread function by holographic methods. We discuss how such approaches can be combined with the use of implantable microlenses and show a first study of holography through them.