Identification of Ablation Zones via Spectral Analysis Following Microwave Treatment of Ex Vivo Liver and Lung

Hyperspectral imaging (HSI) combined with K-means clustering was employed to identify and characterize the most relevant spectral features of the main ablation zones in ex-vivo lamb lung and liver, within the 500-1000 nm wavelength range. Each organ was treated with a clinical microwave ablation (MWA) system at power settings of 15, 30 and 100 W. The average reflectance spectra derived from clustered pixels reveal significant changes in the structural and chemical composition induced by the thermal damage, offering valuable insights for enhancing surgical guidance in thermotherapy.