Bioluminescent mycelium: An exploration into the cultivation of Pannellus stipticus

Mycelium bioluminescence is a fascinating phenomenon produced by certain fungi species. However, optimizing cultivation conditions to achieve constant and high-intensity bioluminescence remains challenging. This research analyses scientific studies on the natural conversion processes of chemicals into light energy of fungi, delving into the optimal conditions for light production. As a result of a series of laboratory experiments and the creation of prototypes, the potential of Pannellus stipticus (Ps) bioluminescence is explored. In particular, the study analyzes a cultivation method for bioluminescent mycelium, highlighting the differences by using various substrates. Results show that the direct application of a Master Culture (MC) in specific substrates significantly increases luminescence’s intensity and duration. The study also quantified, through bioluminescence imaging, the amount of light emitted in lux. In conclusion, after providing a methodology for growing bioluminescent mycelium, this research evaluates the feasibility of using bioluminescent mycelium for outdoor purposes.