Bone marrow (BM) niche is a complex environment made of a stromal and a hematopoietic cellular components. MSCs (mesenchymal stem cells) have a pivotal role in HSCs (hematopoietic stem cells) regulation since they help in preserving their stemness and the balance between self-renewal and differentiation. Such crosstalk is also involved in pathological events: as an example BMSCs can sense peripheral tumour and, by the secretion of IL1β, alter HSC transcription factors activation and guide related differentiation.Organs on chip (OoC) technology can be considered an effective strategy to overcome 2D culture and animal models limitations in modelling such BM niche, by reproducing a highly controlled microenvironment that preserves niche properties in terms of HSCs stemness and differentiation potential. The aim of this study was the development of a functional BM on chip to study the interaction of MSCs on HSCs in a 3D in-vitro microenvironment and at the same time create a model of endosteum niche including multipotent and differentiated MSC
Bone Marrow on chip: unravelling mesenchymal-hematopoietic crosstalk
A. Cordiale;P. Occhetta;M. Rasponi
2023-01-01
Abstract
Bone marrow (BM) niche is a complex environment made of a stromal and a hematopoietic cellular components. MSCs (mesenchymal stem cells) have a pivotal role in HSCs (hematopoietic stem cells) regulation since they help in preserving their stemness and the balance between self-renewal and differentiation. Such crosstalk is also involved in pathological events: as an example BMSCs can sense peripheral tumour and, by the secretion of IL1β, alter HSC transcription factors activation and guide related differentiation.Organs on chip (OoC) technology can be considered an effective strategy to overcome 2D culture and animal models limitations in modelling such BM niche, by reproducing a highly controlled microenvironment that preserves niche properties in terms of HSCs stemness and differentiation potential. The aim of this study was the development of a functional BM on chip to study the interaction of MSCs on HSCs in a 3D in-vitro microenvironment and at the same time create a model of endosteum niche including multipotent and differentiated MSCI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
