Pulsed laser systems, especially those based on fiber technology, employed for SRS microscopy often present excess noise that prevents the user from reaching the shot-noise limit and increases the imaging time. This is detrimental, as SRS is often employed as an alternative to spontaneous Raman when imaging speed is an issue. To compensate for these fluctuations, one can use balanced detection. It employs a second photodiode to monitor the laser fluctuations by splitting a portion of the beam before the sample and subtracts this signal from the one generated at the photodiode after the sample. However, the sample often presents spatially varying transmission, thus unbalancing the two photodiodes. Several solutions to this problem have been proposed and demonstrated in the literature and reviewed in this chapter.
Balanced detection SRS microscopy
Polli, Dario;Cerullo, Giulio
2022-01-01
Abstract
Pulsed laser systems, especially those based on fiber technology, employed for SRS microscopy often present excess noise that prevents the user from reaching the shot-noise limit and increases the imaging time. This is detrimental, as SRS is often employed as an alternative to spontaneous Raman when imaging speed is an issue. To compensate for these fluctuations, one can use balanced detection. It employs a second photodiode to monitor the laser fluctuations by splitting a portion of the beam before the sample and subtracts this signal from the one generated at the photodiode after the sample. However, the sample often presents spatially varying transmission, thus unbalancing the two photodiodes. Several solutions to this problem have been proposed and demonstrated in the literature and reviewed in this chapter.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
