It forms an object of the present invention a micro-droplet plate (I) for the formation, isolation, seeding and controlled recovery of volumes between I and 400 microliters, or between 4 and 50 microliters of fluids, where said fluids are SOL, which comprises: - at least one fixed element (2) which is a rigid flat support which has an upper face (14) and a lower face (15), having, on said upper face (14), wells (4) connected together by microchannels (5), said wells (4) being delimited by a hydrophobic rim (21) and crossed by a hole (6), where said wells ( 4) and said microchannels (5) define at least one microfluidic circuit (16); - moving elements (3), received in said wells (4) through said holes (6), which are cylinders having an upper base (7) and a lower base (8); characterized in that: - said moving elements (3) move, even independently of each other, along their vertical axis (28) and, optionally, around their vertical axis (28); - the upper base (7) of said moving elements (3) comprises a hydrophilic surface with relief items (12). It is a further object a method for the formation, isolation, seeding and/or controlled recovery of drops of gel (17), optionally comprising biological material.

Micro-droplet dish

E. Bianchi;G. A. Dubini;P. De Stefano;
2021

Abstract

It forms an object of the present invention a micro-droplet plate (I) for the formation, isolation, seeding and controlled recovery of volumes between I and 400 microliters, or between 4 and 50 microliters of fluids, where said fluids are SOL, which comprises: - at least one fixed element (2) which is a rigid flat support which has an upper face (14) and a lower face (15), having, on said upper face (14), wells (4) connected together by microchannels (5), said wells (4) being delimited by a hydrophobic rim (21) and crossed by a hole (6), where said wells ( 4) and said microchannels (5) define at least one microfluidic circuit (16); - moving elements (3), received in said wells (4) through said holes (6), which are cylinders having an upper base (7) and a lower base (8); characterized in that: - said moving elements (3) move, even independently of each other, along their vertical axis (28) and, optionally, around their vertical axis (28); - the upper base (7) of said moving elements (3) comprises a hydrophilic surface with relief items (12). It is a further object a method for the formation, isolation, seeding and/or controlled recovery of drops of gel (17), optionally comprising biological material.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1207721
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