Influence of the Stimulation Current on the Differences between Cell and Tissue Electrophysiological Simulations

This study compares stimulation waveforms for single-cell simulations. The alternatives include monophasic and biphasic current pulses, and a new waveform that resembles the transmembrane current responsible for conduction in tissue. Results indicate that the new stimulation produces the lowest mismatch between action potential markers simulated in cell and in tissue. In comparison with the monophasic stimulation, the new stimulation reduced cell-fiber differences by 99% for triangulation, by 95% for maximum transmembrane voltage, and by 76% for the maximum voltage slope. In conclusion, the new stimulation waveform could help to improve the trustworthiness of single-cell simulations in studies involving tissue-derived markers.