Two-photon laser writing is used here to fabricate 3D proteinaceous microstructures with photothermal functionality in the near-infrared spectral region and tunable elasticity. The photo-cross-linking is initiated in bovine serum albumin (BSA) by rose bengal or methylene blue and the photo-thermal effect arises from gold non-spherically symmetric nanoparticles dispersed in the ink. Massive energy transfer of the plasmonic resonances of the gold nanoparticles to methylene blue prevents effective photo-crosslinking of BSA. However, stable microstructures with photo-thermal functionality can be fabricated in the rose bengal proteinaceous inks. On these microstructures, with a gold atom concentration as low as 1% w/w, a highly localized temperature increase can be quickly (≅1 s) reached and maintained under continuous wave laser irradiation at 800 nm. The photothermal efficiency under continuous wave laser irradiation depends on the thickness of the microstructure and can reach 12.2 ± 0.4 °C W−1 These proteinaceous microstructures represent therefore a promising platform for future applications in the fields like physical stimulation of cells for regenerative nanomedicine.

Multiphoton Fabrication of Proteinaceous Nanocomposite Microstructures with Photothermal Activity in the Infrared

Polli, Dario;De la Cadena, Alejandro;
2020-01-01

Abstract

Two-photon laser writing is used here to fabricate 3D proteinaceous microstructures with photothermal functionality in the near-infrared spectral region and tunable elasticity. The photo-cross-linking is initiated in bovine serum albumin (BSA) by rose bengal or methylene blue and the photo-thermal effect arises from gold non-spherically symmetric nanoparticles dispersed in the ink. Massive energy transfer of the plasmonic resonances of the gold nanoparticles to methylene blue prevents effective photo-crosslinking of BSA. However, stable microstructures with photo-thermal functionality can be fabricated in the rose bengal proteinaceous inks. On these microstructures, with a gold atom concentration as low as 1% w/w, a highly localized temperature increase can be quickly (≅1 s) reached and maintained under continuous wave laser irradiation at 800 nm. The photothermal efficiency under continuous wave laser irradiation depends on the thickness of the microstructure and can reach 12.2 ± 0.4 °C W−1 These proteinaceous microstructures represent therefore a promising platform for future applications in the fields like physical stimulation of cells for regenerative nanomedicine.
2020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1166494
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