In this report, we discuss the interest of quality metrics for imaging and image processing of multi-views in light sheet fluorescent 3D microscopy. Various metrics of focus are tested on real and simulated data so as to automatically assess the informational quality of the images. Application of such metrics are given for several information tasks including online control of acquisition, fast registration or image fusion. Illustrations are given for typical samples of interest for in vivo imaging with light sheet microscopy such as spheroids or organoids. We point to the reader softwares freely available under Fiji which enable to test the computation of a basic quality metric, for registration and fusion.

On the use of image quality measures of multi-views in light sheet fluorescence 3D microscopy

Bassi A.;ROUSSEAU, DAVID MARC MICHEL
2018-01-01

Abstract

In this report, we discuss the interest of quality metrics for imaging and image processing of multi-views in light sheet fluorescent 3D microscopy. Various metrics of focus are tested on real and simulated data so as to automatically assess the informational quality of the images. Application of such metrics are given for several information tasks including online control of acquisition, fast registration or image fusion. Illustrations are given for typical samples of interest for in vivo imaging with light sheet microscopy such as spheroids or organoids. We point to the reader softwares freely available under Fiji which enable to test the computation of a basic quality metric, for registration and fusion.
2018
Proceedings of SPIE - The International Society for Optical Engineering
9781510618800
9781510618817
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1090914
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