The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.

Fluorescence lifetime imaging of intracellular magnesium content in live cells

Candeo, Alessia;D'Andrea, Cosimo;Valentini, Gianluca;Taroni, Paola;
2019-01-01

Abstract

The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.
2019
Biochemistry; Fluorescence, Imaging, Magnesium
File in questo prodotto:
File Dimensione Formato  
iotti new sub_rev PT.pdf

Open Access dal 02/03/2020

Descrizione: Post-print
: Post-Print (DRAFT o Author’s Accepted Manuscript-AAM)
Dimensione 564.08 kB
Formato Adobe PDF
564.08 kB Adobe PDF Visualizza/Apri
Sargenti_Analyst2019.pdf

Accesso riservato

: Publisher’s version
Dimensione 1.17 MB
Formato Adobe PDF
1.17 MB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1080003
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 2
social impact