The results presented in this contribution stem out a collaborative interdisciplinary effort aimed at identifying the most suitable imaging techniques to map the distribution of functionalized nanoparticles acting as biological markers taken up by specific cellular receptors in healthy and pathological biological tissues. Nanoparticles can be labelled to hypoxia seeking agents or other tumour bio-parameters; X-rays can then be used to probe the markers at depth, with contrast given by the nanoparticles. The uptake of nanoparticles by cancer cells could also enable detection of small clusters of infiltrating cancer cells, which are currently missed by commonly used imaging modalities. We have already demonstrated in previous works the feasibility of use of a L-XRF system in measuring gold NP concentrations and this work will focus on the development and validation of a K-XRF detection system in order to ease penetration through overlying tissue. The K-absorption edges of Gold, Gadolinium and Silver are conveniently located (80.7 keV, 50.2 keV and 25.5 keV, respectively) so we developed a custom compact detection module, which accommodates a CdTe pn diode detector (4 × 4 mm active area) and all the needed biasing and frontend electronics. The detection module is conceived to allow the possibility of coupling mechanical collimators or X-ray poly-capillary lenses for high energy to perform 2D imaging. The contribution focuses on the advantages of the use of different types of NPs in cancer imaging and on the results of the experimental qualification carried out with different NPs (Gold, Gadolinium and Silver) and samples to assess the technique detection limit, linearity and sensitivity as a function of the NP material.

Multi-color Imaging of NP in Phantoms towards Theranostic Imaging of Tumours

Andrea Castoldi;Chiara Guazzoni;
2018-01-01

Abstract

The results presented in this contribution stem out a collaborative interdisciplinary effort aimed at identifying the most suitable imaging techniques to map the distribution of functionalized nanoparticles acting as biological markers taken up by specific cellular receptors in healthy and pathological biological tissues. Nanoparticles can be labelled to hypoxia seeking agents or other tumour bio-parameters; X-rays can then be used to probe the markers at depth, with contrast given by the nanoparticles. The uptake of nanoparticles by cancer cells could also enable detection of small clusters of infiltrating cancer cells, which are currently missed by commonly used imaging modalities. We have already demonstrated in previous works the feasibility of use of a L-XRF system in measuring gold NP concentrations and this work will focus on the development and validation of a K-XRF detection system in order to ease penetration through overlying tissue. The K-absorption edges of Gold, Gadolinium and Silver are conveniently located (80.7 keV, 50.2 keV and 25.5 keV, respectively) so we developed a custom compact detection module, which accommodates a CdTe pn diode detector (4 × 4 mm active area) and all the needed biasing and frontend electronics. The detection module is conceived to allow the possibility of coupling mechanical collimators or X-ray poly-capillary lenses for high energy to perform 2D imaging. The contribution focuses on the advantages of the use of different types of NPs in cancer imaging and on the results of the experimental qualification carried out with different NPs (Gold, Gadolinium and Silver) and samples to assess the technique detection limit, linearity and sensitivity as a function of the NP material.
2018
2018 IEEE Nuclear Science Symposium and Medical Imaging Conference Records
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1073166
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