We report the fabrication and validation of a microfluidic chip for fluorescence detection, which incorporates in the same glass substrate the microfluidic network, the excitation, the filtering, and the collection elements. The device is fabricated in a hybrid approach combining different technologies, such as femtosecond laser micromachining and RF sputtering, to increase their individual capabilities. The validation of the chip demonstrates a good wavelength selective light filtering and a limit of detection of a 600-nM concentration of Oxazine 720 perchlorate dye.

Highly integrated lab-on-a-chip for fluorescence detection

SCOTOGNELLA, FRANCESCO;RAMPONI, ROBERTA;LANZANI, GUGLIELMO;
2016-01-01

Abstract

We report the fabrication and validation of a microfluidic chip for fluorescence detection, which incorporates in the same glass substrate the microfluidic network, the excitation, the filtering, and the collection elements. The device is fabricated in a hybrid approach combining different technologies, such as femtosecond laser micromachining and RF sputtering, to increase their individual capabilities. The validation of the chip demonstrates a good wavelength selective light filtering and a limit of detection of a 600-nM concentration of Oxazine 720 perchlorate dye.
2016
femtosecond laser; fluorescence; optofluidics, binary Fresnel lens, femtosecond laser microfabrication, photonic crystal; Atomic and Molecular Physics, and Optics; Engineering (all)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11311/1014467
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