Chemo-enzymatic approach to the synthesis of each of the four isomers of α-alkyl-β-fluoroalkyl-substituted β-amino acids

Starting from easily available ethyl 2-methyl-4,4,4-trifluoroacetoacetate and benzylamine each of the four stereoisomers of α-methyl-β-trifluoromethyl-β-alanine have been synthesized in optically pure form via stereocontrolled chemo-enzymatic procedure including diastereoselective base-catalyzed [1,3]-proton shift reaction and enantioselective penicillin acylase-catalyzed resolution. Each of the four stereoisomers of α-methyl-β-trifluoromethyl-β-alanine have been synthesized via stereocontrolled chemo-enzymatic approach.